Would the white king have to give up opposition at some point to take the key squares?
Yes. When you're defending using the opposition, you want to keep it. When you're attacking using the opposition, you usually have to give it up to attain your objectives (a bypass manouver).
1. Ka2? throws away the win after 1... Kd4!
Now White has a choice: keep the opposition, but stay on the second rank or bypass and give up the opposition.
Staying on the second rank gets nowhere: 2. Kb2 Ke4 3. Kc2 Kd4 4. Kd2 Ke4 5. Ke2 Kd4 6. Kf2 Ke4.
Bypassing doesn't help either: 5. Kc3 Ke5 6. Kc4 (6.Kd3 Kd5) Ke4 7. Kc5 Ke5 8. Kc6 Ke6 etc.
White needs to play 1. Ka4! and bypass further up the board.
I am just beginning to learn king and pawn endings so please bare with me for what might seem like stupid questions. In the position below, White to move can win, but black to move can defend. My questions are, if it's white to move, there are two options to take the opposition, the direct distant opposition of a4, or the virtual opposition of a2. Does it matter which opposition is taken? And if he goes to A4, will he then have to take the virtual opposition at some point anyway? Say, 1. A4 e5 2 a3? Assuming best play by black with white to move first, can someone explain what the best play from white would be move by move to reach the key squares and why? Would the white king have to give up opposition at some point to take the key squares?